Probiotic strains from Lactobacillus salivarius and antimicrobial agents obtained therefrom

US7186545B2 · kind B2 · utility

Assignees

• Enterprise Ireland · IE
• UNIVERSITY COLLEGE CORK—NATIONAL UNIVERSITY OF IRELAND, Cork · IE

Inventors

• John Collins · Cork, IE
• Gerald O'Sullivan · Thurles, IE
• Gerardine Mary Thornton · Ballincollig, IE
• Marian Mary Geraldine O'Sullivan · Blessington, IE

Key dates

• Filing date: Jun 26, 2003
• Grant date: Mar 6, 2007
• Expiry date: Apr 4, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12R2001/225
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

A strain of Lactobacillus salivarius isolated from resected and washed human gastrointestinal tract inhibits a broad range of Gram positive and Gram negative microorganisms and secretes a product having antimicrobial activity into a cell-free supernatant. The activity is produced only by, growing cells and is destroyed by proteinase K and pronase E, the inhibitory properties of the strain and its secretory products being maintained in the presence of physiological concentrations of human bile and human gastric juice. The strain exhibits a broad-spectrum of activity against bacteria including Listeria, Staphylococcus, including methocillin resistant St. aureus (MRSA), and Bacillus, but does not inhibit many closely related lactobacilli. An antimicrobial agent is obtained from the strain which has bacteriocin-like properties.