Biomolecules having multiple attachment moieties for binding to a substrate surface

US7186813B1 · kind B1 · utility

Assignee

• Nanogen Recognomics GmbH · DE

Inventors

• Markus Schweitzer · Frankfurt am Main, DE
• Norbert Windhab · Hofheim, DE
• John Havens · San Diego, US
• Thomas J. Onofrey · San Marcos, US
• Charles Greef · Louisville, US
• Daguang Wang · White Plains, US

Key dates

• Filing date: Aug 11, 2000
• Grant date: Mar 6, 2007
• Expiry date: Jan 28, 2022

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C40B40/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Biomolecules are provided having multiple binding sites for attachment to a substrate surface. The multiple attachment sites may be produced directly on the biomolecule or through use of branched phosphoramidite moieties that can be added in multiple to form dendritic structures which can in turn provide attachment sites for substrate binding moieties. Substrate binding moieties may include noncovalent binding moieties. For covalent binding moieties oligonucleotides containing hydrazides are provided. These hydrazides can be introduced via protected building blocks such as phosphoramidites or via building blocks containing precursor forms of such hydrazides.