Array-based epitope-captured antibody display
US7192720B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 27, 2004 |
| Grant date | Mar 20, 2007 |
| Priority date | — |
| Expiry date | Oct 11, 2024 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2600/00
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Reagents and methods for detecting target proteins in a sample are provided. The reagents include a replicable genetic package, a protein displayed on an exterior surface of the package that is expressed from a heterologous nucleic acid borne by the package, and one or more antibodies complexed with the expressed protein and which have an open binding site for a target protein. Thus, a segment of the nucleic acid encodes for an epitope that is shared by the expressed polypeptide and the target protein. The reagents can be utilized individually or as part of a library or an array to bind target proteins within protein samples to form one or more complexes. By determining the sequence of the segment of the heterologous nucleic acid of a package within a complex, one can identify the target protein since the segment encodes for an epitope that is shared by the expressed and target proteins.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.