Biosynthesis of amorpha-4,11-diene
US7192751B2 · kind B2 · utility
22Cited by
11References
33Claims
0Family size
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Key dates
| Filing date | Apr 9, 2003 |
| Grant date | Mar 20, 2007 |
| Priority date | — |
| Expiry date | Apr 9, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P5/007
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods for synthesizing amorpha-4,11-diene synthase from isopentenyl pyrophosphate are provided. A first method comprises introducing into a host microorganism a plurality of heterologous nucleic acid sequences, each coding for a different enzyme in the mevalonate pathway for producing isopentenyl pyrophosphate. Amorpha-4,11-diene synthase is then produced using an optimized amorpha-4,11-diene synthase gene. The invention also provides nucleic acid sequences, enzymes, expression vectors, and transformed host cells for carrying out the methods.
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