Method for purification of recombinant proteins
US7193041B2 · kind B2 · utility
6Cited by
2References
8Claims
0Family size
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Key dates
| Filing date | Apr 19, 2001 |
| Grant date | Mar 20, 2007 |
| Priority date | — |
| Expiry date | Feb 16, 2024 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N30/02
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
Purification of poly-amino acid-tagged recombinant proteins has been improved by the use of a carboxymethylated aspartate ligand complexed with a third-block transition metal having an oxidation state of 2+ and a coordination number of 6. A method for synthesizing the metal ion-CM-Asp complex is also described. Further, the metal ion-CM-Asp complex can be used for screening protein function.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.