Compositions to identify swine genetically resistant to F18 E. coli associated diseases

US7193072B2 · kind B2 · utility

Assignees

• Biotechnology Research and Development Corporation · US
• The United States of America, as represented by the Secretary of Agriculture · US
• Swiss Federal Institute of Technology · CH

Inventors

• Brad T. Bosworth · Roanoke Rapids, US
• Peter Vogeli · Seattle, US

Key dates

• Filing date: Jul 25, 2001
• Grant date: Mar 20, 2007
• Expiry date: Nov 14, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention provides non-invasive methods and compositions to differentiate, with a high level of sensitivity and specificity, swine that are genetically susceptible to diseases associated with F18 E. coli infection, from resistant swine. DNA polymorphisms in the swine alpha (1,2) fucosyltransferase 1 (FUT1) gene were used to differentiate resistant from susceptible swine. The invention includes a polypeptide with amino acid substitutions, encoded by the nucleotide polymorphisms, a molecular diagnostic assay, and a kit for the differentiation, of E. coli F18-adhesion resistant, heterozygous (carrier) and homozygous susceptible pigs. The molecular test identifies susceptibility to oedema disease and postweaning diarrhea with high sensitivity and specificity, therefore, is useful to swine breeder in their effort to enhance for resistance. Information on the polymorphisms of the present invention provides insight into causation and treatment of E. coli associated intestinal disorders.