Oligonucleotide pairs for multiplexed binding assays
US7195875B2 · kind B2 · utility
6Cited by
12References
24Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Apr 18, 2003 |
| Grant date | Mar 27, 2007 |
| Priority date | — |
| Expiry date | Sep 14, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6837
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention is an oligonucleotide-based assay system and kit useful for sorting, detecting, and identifying analytes. The system utilizes complementary oligonucleotide pairs in which one oligonucleotide of each pair is immobilized to a solid substrate and the other oligonucleotide has an analyte binding agent attached to it. The different oligonucleotide pairs hybridize at substantially the same rate, have substantially the same Tm, have nucleotide sequences designed to minimize cross-hybridization between different pairs, and hybridize together relatively rapidly at ambient temperatures without detectable cross-hybridization.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.