Methods for controlling gibberellin levels

US7195917B2 · kind B2 · utility

Assignee

• Monsanto Technology LLC · US

Inventors

• Sherri M. Brown · Chesterfield, US
• Tedd D. Elich · Durham, US
• Gregory R. Heck · Crystal Lake Park, US
• Ganesh M. Kishore · Chesterfield, US
• Eugene W. Logusch · Chesterfield, US
• Sherry J. Logusch · Chesterfield, US
• Kenneth J. Piller · Davidson, US
• Sudabathula Rao · Chesterfield, US
• Joel E. Ream · Chesterfield, US
• Scott R. Baerson · Oxford, US

Key dates

• Filing date: Mar 27, 2003
• Grant date: Mar 27, 2007
• Expiry date: Sep 15, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8297
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods and materials are disclosed for the inhibition and control of gibberellic acid levels. In particular, nucleic acid sequences of copalyl diphosphate synthase. 3-β hydroxylase, and 2-oxidase and additional nucleic acid sequences are disclosed. Gibberellic acid levels may be inhibited or controlled by preparation of a chimeric expression construct capable of expressing a RNA or protein product which suppresses the gibberellin biosynthetic pathway sequence, diverts substrates from the pathway or degrades pathway substrates or products. The sequence is preferably a copalyl diphosphate synthase sequence, a 3β-hydroxylase sequence, a 2-oxidase sequence, a phytoene synthase sequence, a C20-oxidase sequence, and a 2β,3β-hydroxylase sequence. Administration of a complementing agent, preferably a gibberellin or gibberellin precursor or intermediate restores bioactivity.