Late-PCR

US7198897B2 · kind B2 · utility

Assignee

• BRANDEIS UNIVERSITY · US

Inventors

• Lawrence J. Wangh · Waltham, US
• Kenneth Pierce · Natick, US
• Cristina Hartshorn · Dedham, US
• John Rice · Deseronto, CA
• J. Aquiles Sanchez · Framingham, US

Key dates

• Filing date: Dec 17, 2002
• Grant date: Apr 3, 2007
• Expiry date: May 27, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6851
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A non-symmetric polymerase chain reaction (PCR) amplification method employing a limiting primer in low concentration whose concentration-adjusted melting point at least equals, and preferably exceeds, that of the excess primer, the latter in turn not being more than 25° C. below the melting temperature of the amplicon. Assays employing such amplification and labeled hybridization probes, including assays that include a detection step following primer extension or a low-temperature probe, or both. Kits for performing such assays and primer or primer-and-probe sets for performing the foregoing amplifications and assays.