Bioreactor apparatus and cell culturing system
US7198940B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 25, 2001 |
| Grant date | Apr 3, 2007 |
| Priority date | — |
| Expiry date | Oct 25, 2021 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/809
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A bioreactor apparatus and cell culturing system is provided for the automated cultivation and processing of living cells remotely both on earth and in low gravity which utilizes a generally cylindrical reactor vessel that may be optionally rotated about its cylindrical axis while allowing the entrance of fresh or recycled fluid and the removal, optionally, of spent medium, medium to be recycled or filtered or unfiltered medium for the collection of samples. A method of exchanging gases between the culture medium and ambient gases is provided. A fresh-medium storage bag and peristaltic pump is used for batch feeding, perfusion or sample collection. An enclosure and manifold representing an additional level of chemical containment and a series of pinch valves for the periodic collection of samples of suspended cells or cell-free medium is disposed therein together with a humidity control system. The bioreactor is computer controlled in order to control all functions including rotation of the reactor vessel, feeding fresh medium, perfusing the reactor vessel, timed collection of samples of fluid from the reactor, selecting between collecting cells or cell-free supernatant. A sealed c…
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.