Methods and compositions for obtaining mature dendritic cells
US7198948B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jan 15, 2002 |
| Grant date | Apr 3, 2007 |
| Priority date | — |
| Expiry date | Jan 15, 2022 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2502/11
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
We describe an improved method for generating sizable numbers of mature dendritic cells from nonproliferating progenitors in human blood. The first step or “priming” phase is a culture of T cell depleted mononuclear cells in medium supplemented with GM-CSF and IL-4 to produce immature dendritic cells. The second step or “differentiation” phase requires the exposure to dendritic cell maturation factor such as monocyte conditioned medium. Using this two-step approach, substantial yields are obtained. The dendritic cells derive from this method have all the features of mature cells. They include a stellate cell shape, nonadherence to plastic, and very strong T cell stimulatory activity. The mature dendritic cells produced according to this invention are useful for activating T cells.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.