Optical analytic measurement device for fluorescence measurements in multisample carriers

US7199377B2 · kind B2 · utility

Assignee

• Cybio AG · DE

Inventors

• Juergen Wulf · Helmstedt, DE
• Eberhard Dietzsch · Hermsdorf, DE
• Thomas Moore · Jena, DE

Key dates

• Filing date: Apr 1, 2005
• Grant date: Apr 3, 2007
• Expiry date: Sep 30, 2025

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2021/6463
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention is directed to a device for optical analytic measurement in a multisample carrier, particularly for measuring fluorescence or bioluminescence. The object of the invention, to find a novel possibility for measuring fluorescence or luminescence in multisample carriers, wherein, during excitation of all of the wells, the secondary radiation of each well is measured simultaneously without impermissible contributions of background radiation which falsify the characteristic emission of the sample material, is met according to the invention in that the excitation light from a light source unit is directed on the multisample carrier coaxially in a ring-shaped manner around an optical axis wherein the optical axis is oriented in direction of a surface normal of the multisample carrier and coaxial to the direction of the readout beam path, and a ring mirror unit with at least one curved ring mirror, which is arranged coaxial to the optical axis in such a way that the excitation light; illuminates the multisample carrier homogeneously on all sides at an oblique incident angle, is provided for orientation of the excitation light on the multisample carrier.