Isolated protein having hydroxynitrile lyase activity

US7202075B2 · kind B2 · utility

Assignee

• DSM Fine Chemicals Austria NFG GmbH & Co KG · AT

Inventors

• Helmut Schwab · Graz, AT
• Anton Glieder · Gleisdorf, AT
• Christoph Kratky · Graz, AT
• Ingrid Dreveny · Graz, AT
• Rodolfo Bona · Graz, AT
• Peter Pochlauer · Dordrecht, NL
• Wolfgang Skranc · Wien, AT
• Herbert Mayrhofer · Linz, AT
• Irma Wirth · Ansfelden, AT
• Rudolf Neuhofer · Mittertreffling, AT

Key dates

• Filing date: Sep 15, 2004
• Grant date: Apr 10, 2007
• Expiry date: Dec 23, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/52
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

New genes containing a DNA sequence coding for hydroxynitrile lyase, which genes can be prepared via a primer combination based on the DNA sequence of the 5′-region of the mdl genes from the Prunus serotina and from Prunus amygdalus and/or a primer 2 based on the 3′-region of the DNA sequences of one of the hydroxynitrile lyase isoenzymes from Prunus serotina or from Prunus amygdalus, subsequent amplification with a DNA polymerase using a DNA from organisms, containing genes coding for hydroxynitrile lyase, as templates and cloning, and also recombinant proteins which can be prepared in suitable host cells by heterologous expression of the DNA sequence of said HNL genes, and proteins and fusion proteins derived therefrom and use of said proteins for preparing (R)- or (S)-cyanohydrins.