Methods for reducing background fluorescence
US7205100B2 · kind B2 · utility
9Cited by
5References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Oct 9, 2003 |
| Grant date | Apr 17, 2007 |
| Priority date | — |
| Expiry date | Sep 9, 2024 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S435/962
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention relates to methods for reducing background fluorescence in cell detection and measurement systems. In accordance with one aspect of the invention, cell-impermeant chemical agents are used to modify the fluorescent probes that reside outside the cells to be detected. The chemical agents can chemically modify one or more functional groups in the probes, thereby reducing or eliminating the fluorescence of the probes. In accordance with another aspect of the invention, Acid Black 48 is used to reduce background fluorescence produced by fluorescent probes such as SYTO 62.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.