Multi-segment cartridge for bio-separation with multiplexed fluorescence detection
US7208072B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 21, 2003 |
| Grant date | Apr 24, 2007 |
| Priority date | — |
| Expiry date | Feb 7, 2024 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44782
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A bio-separation instrument using a multi-segment cartridge. The cartridge includes a plurality of efficient, compact, portable, interchangeable, reusable, recyclable, modular, multi-channel segments. Each segment supports multiple capillaries for CE separation. Each segment has an integrated reservoir containing a buffer/gel common to all the capillaries. Air pressure can be supplied to the reservoir through the periphery of each segment. Each segment has an integrated LED array board directly coupled to capillaries for providing excitation light. Optical fibers of a fiber bundle are individually guided through the periphery of each segment to the capillaries for emission collection. The fiber bundle delivers emissions to a detector that can read the emissions in a time-staggered multiplexed scheme. Each segment includes electrodes electrically coupled to a voltage source through the periphery of the segment for effecting CE separation. Providing each segment with pneumatic and voltage connections on the periphery and direct coupling LED's allow the segments to easily stack together for integration into the instrument.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.