Chromatographic two-layer particles
US7208093B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 18, 2003 |
| Grant date | Apr 24, 2007 |
| Priority date | — |
| Expiry date | May 11, 2024 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J41/20
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The present invention is a method of producing a chromatographic separation matrix, wherein porous polymeric particles comprised of two layers with different properties are prepared in a two-phase system by (a) providing porous polymeric particles with reactive groups on their surfaces; (b) washing said particles with a first solvent and draining the particles to enclose a first phase; (c) wetting the outer layer of the particles by adding a second solvent, which is essentially insoluble in the first solvent; (d) reacting the reactive groups in the outer layer by adding a reagent, which is essentially non-reactive in the first solvent; and (e) coupling of chromatographic binding groups to the reactive groups in the inner layer. The invention also encompasses a porous polymer particle suitable for use as a chromatographic separation matrix and a process of separation, wherein a matrix according to the invention is used.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.