Method for labeling DNA and RNA
US7208269B2 · kind B2 · utility
6Cited by
3References
15Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 23, 2002 |
| Grant date | Apr 24, 2007 |
| Priority date | — |
| Expiry date | Jul 4, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6816
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for fragmenting and labeling nucleic acids is provided. The method comprises maintaining double- and single-stranded nucleic acid molecules in an aerobic or an anaerobic atmosphere, contacting the molecules with hydrogen peroxide and radical generating coordination complexes for a time and at concentrations sufficient to produce aldehyde moieties on the molecules, reacting the aldehyde moieties with amine to produce a condensation product, and labeling the condensation product.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.