Compositions and methods for nucleic acid extraction from biological samples
US7214484B2 · kind B2 · utility
30Cited by
3References
11Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 17, 2002 |
| Grant date | May 8, 2007 |
| Priority date | — |
| Expiry date | Mar 26, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Methods and compositions for extracting nucleic acids from a biological sample are provided. The extraction compositions contain a protease enzyme such as proteinase K at alkaline pH with little or no surfactant present. Extraction can be efficiently performed in 60 minutes or less at room temperature for certain mammalian tissue samples and at elevated temperatures for certain plant tissues.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.