Method for coarse purification of cell digests from microorganisms
US7214508B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 20, 2003 |
| Grant date | May 8, 2007 |
| Priority date | — |
| Expiry date | Feb 20, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/1003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the coarse clarification of lysed cell material from microorganisms and particularly to a method of obtaining nucleic acids. In certain embodiments, the present invention provides a method of coarsely clarifying cell lysate from microorganisms that comprises: lysing the microorganisms under the normal atmospheric pressure to form a flocculent precipitate in the lysate, compressing the flocculent precipitate in the lysate by reducing or increasing the pressure of the atmosphere surrounding the lysate in relation to the normal atmospheric pressure to form a compressed flocculent phase and a liquid phase, and separating the two phases.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.