Screening method for identifying heme independent modulators of soluble guanylyl cyclase (sGC) activity using αβCys105 mutant sGC
US7214519B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Feb 11, 2004 |
| Grant date | May 8, 2007 |
| Priority date | — |
| Expiry date | Feb 6, 2025 |
Classification
- Technology area (CPC A)Human Necessities
- CPC primaryA61K38/00
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of screening a substance of interest for heme independent modulation of enzymatic activity of soluble guanylyl cyclase (sGC) is disclosed, comprising (a) obtaining αβCys105 mutant sGC enzyme; (b) determining activity of the mutant enzyme for forming cGMP from GTP in the presence of the substance of interest in a reaction medium; (c) determining activity as in step (b), except in the absence of the substance of interest; optionally, (d) including an activator other than the substance of interest in steps b) and c); e) comparing results of (b)–(d) to yield a comparison result; and f) from that value of that result, assessing activity of the substance of interest for modulating cGMP production by the mutant enzyme. Increased or decreased formation of cGMP in the presence of the substance of interest indicates activity of the substance for modulating heme independent cGMP production.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.