Nucleotide-sugar-synthesizing enzymes from nonparasitic protists

US7217550B2 · kind B2 · utility

Assignee

• Nutrinova Nutrition Specialist & Food Ingredients GmbH · DE

Inventors

• Thomas Kiy · Frankfurt, DE
• Lothar Elling · Aachen, DE
• Maria-Regina Kula · Jülich, DE

Key dates

• Filing date: May 12, 2003
• Grant date: May 15, 2007
• Expiry date: Aug 2, 2024

Classification

• Technology area (CPC Y): Emerging Cross-Sectional Technologies
• CPC primary: Y10S435/947
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present invention relates to nucleotide-sugar-synthesizing enzymes (enzymes with nucleotidyltransferase or pyrophosphorylase activity) from nonparasitic protists, to a process for the preparation thereof and to the use thereof for preparing nucleotide-sugars. The enzymes according to the invention make possible or greatly simplify the enzymatic preparation of various nucleotide-sugars on the industrial scale from low-cost precursors. It is possible with the aid of the discovered enzymes to prepare, for example, GDP-fucose, GDP-mannose, UDP-glucose, UDP-glucosamine, UDP-galactose, UDP-galactosamine, UDP-N-acetylglucosamine and UDP-N-acetylgalactosamine in economic quantities.