Extraneous DNA sequence that facilitates hantavirus gene expression

US7217812B2 · kind B2 · utility

Assignee

• The United States of America as represented by the Secretary of the Army · US

Inventors

• Jay Hooper · New Market, US
• Connie Schmaljohn · Frederick, US
• Max Custer · Durham, US

Key dates

• Filing date: Mar 21, 2003
• Grant date: May 15, 2007
• Expiry date: Nov 24, 2024

Classification

• Technology area (CPC A): Human Necessities
• CPC primary: A61K2039/53
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

In this application is described a protective DNA vaccines against infection with HFRS- and HPS-associated hantaviruses. The vaccines were constructed by subcloning cDNA representing the medium (M) (encoding the G1 and G2 glycoproteins) into the DNA expression vector pWRG7077. Animals vaccinated with the M construct developed a neutralizing antibody response. Passive transfer experiments show that serum from vaccinated animals, when injected on days 4 or 5 after challenge, protected animals from lethal disease.