Bifunctional molecules and their use in the disruption of protein-protein interactions

US7220552B1 · kind B1 · utility

Assignee

• The Board of Trustees of the Leland Stanford Junior University · US

Inventors

• Gerald R. Crabtree · Woodside, US
• Kryn Stankunas · Menlo Park, US
• Roger Briesewitz · Mountain View, US
• Thomas J. Wandless · Cambridge, US

Key dates

• Filing date: Nov 17, 2000
• Grant date: May 22, 2007
• Expiry date: Nov 17, 2020

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/542
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Bifunctional inhibitor molecules and methods for their use in the inhibition of protein—protein interactions are provided. The subject bifunctional inhibitor molecules are conjugates of a target protein ligand and a blocking protein ligand, where these two moieties are optionally joined by a linking group. In the subject methods, an effective amount of the bifunctional inhibitor molecule is administered to a host in which the inhibition of a protein—protein interaction is desired. The bifunctional inhibitor molecule simultaneously binds to its corresponding target and blocking proteins to produce a tripartite complex that inhibits the target protein—protein interaction. The subject methods and compositions find use in a variety of applications, including therapeutic applications.