Method for orthogonal analyte stacking/injection systems in electrophoresis
US7223325B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 19, 2001 |
| Grant date | May 29, 2007 |
| Priority date | — |
| Expiry date | Jun 1, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N27/44743
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
In the present capillary electrokinetic chromatograpy method, analytes are injected by electroosmotic flow directly from a sample matrix into a separation buffer containing an electrokinetic vector with an opposite mobility. Analytes can now be injected at the velocity of electroosmotic flow, but are retained at the interface of the sample matrix-co-ion and separation buffer micelle zones as analyte/micelle complexes. Manipulation of the injecting force and opposing stacking force allow greatly increased length or volume of injection. Concentrations of the micelle, methanol, and borate in the separation buffer were provided to increase maximum injection length of neutral analytes. Reducing the analyte velocity in the separation buffer without substantially decreasing the velocity of the analyte during injection from the sample vial allowed greatly extended sample plug injection lengths. It is further enabled to inject sample solvent volumes equivalent to about twenty times the effective capillary volume. Equations and algorithms describing the injection process and maximum injection lengths for this mode of stacking in electrokinetic capillary chromatography are introduced. Use of …
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.