Identification of 3-ketosteroid 9-alfa-hydroxylase genes and microorganisms blocked in 3-ketosteroid 9-alfa-hydroxylase activity
US7223579B2 · kind B2 · utility
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Key dates
| Filing date | Feb 19, 2003 |
| Grant date | May 29, 2007 |
| Priority date | — |
| Expiry date | May 20, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P33/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to an isolated polynucleotide sequence comprising a nucleic acid sequence encoding the amino acid sequence of KshA protein or of KshB protein, encoded by nucleotides 499–1695 of SEQ ID NO: 1 or by nucleotides 387–1427 of SEQ ID NO:2, respectively, and functional homologues thereof. The polynucleotides of the invention can be used to construct genetically modified microorganisms blocked in 3-ketosteroid 9α-hydroxylase activity, which are useful in the microbial degradation of steroids to accumulate certain steroid products.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.