Process for the preparation of phosphorothioate oligonucleotides
US7227017B2 · kind B2 · utility
50Cited by
26References
37Claims
0Family size
Assignee
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Key dates
| Filing date | Jun 5, 2003 |
| Grant date | Jun 5, 2007 |
| Priority date | — |
| Expiry date | Dec 27, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07H21/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
A process for the synthesis of phosphorothioate oligonucleotides is provided which comprises assembling an oligonucleotide bound to a solid support in the presence of acetonitrile; prior to cleaving the oligonucleotide from the solid support removing the acetonitrile; and cleaving the oligonucleotide from the solid support. The process is particularly suited to the large scale synthesis of nucleotides. The acetonitrile may be removed from the solid support by one or both of drying and by washing with solvents. Preferred washing solvents comprise trialkylamines.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.