Transposon mediated multiplex sequencing
US7229798B2 · kind B2 · utility
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16Claims
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Key dates
| Filing date | Jul 5, 2001 |
| Grant date | Jun 12, 2007 |
| Priority date | — |
| Expiry date | Feb 6, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6869
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to an automated method of transposon-mediated multiplex sequencing of DNA fragments inserted into a vector. It relates more particularity to an increased efficiency in such automated methods, where the increased efficiency is obtained by screening out before the sequencing those constructs in which the transposon inserted into the vector sequence. This prevents a waste of time and resources in performing reactions sequencing the vector instead of the DNA fragments of interest.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.