Double-transfected cell line useful for the identification of transport inhibitors

US7229825B2 · kind B2 · utility

Assignee

• DEUTSCHES KREBSFORSCHUNGSZENTRUM STIFTUNG DES ÖFFENTLICHEN RECHTS · DE

Inventors

• Yunhai Cui · Heidelberg, DE
• Joerg Koenig · Griesheim, DE
• Dietrich Keppler · Dossenheim, DE

Key dates

• Filing date: Dec 5, 2003
• Grant date: Jun 12, 2007
• Expiry date: Apr 16, 2024

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/00
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Described is a double-transfected cell line containing (a) a DNA sequence encoding an uptake transporter for organic anions, preferably OATP8, operatively linked with a promoter and (b) a DNA sequence encoding an export pump for organic anions or anionic conjugates, preferably the multidrug resistance protein 2 (MRP2), operatively linked with a promoter. Moreover, various uses of said cell line are described, preferably its use for the identification of transport inhibitors, e.g. drug candidates.