Patent · US Expired

Preparation containing cell extracts for cell-free protein synthesis and means for synthesizing protein using the preparation

US7235382B2 · kind B2 · utility

9Cited by
1References
11Claims
0Family size

Assignee

Inventors

Key dates

Filing dateMar 25, 2005
Grant dateJun 26, 2007
Priority date
Expiry dateMar 25, 2025

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY02P20/582
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Disclosed are a preparation containing cell extracts for cell-free protein synthesis, prepared by excluding from a living organism a system, participating to inhibiting of self protein synthesis reaction, an apparatus for cell-free protein synthesis reaction equipped with a reaction tank for cell-free protein synthesis, and a kit for use therefor; the preparation can be stored at room temperature and prepared as a preparation in a state where biological functions of the cell extracts are maintained, and further, disclosed is methods for cell-free protein synthesis comprising providing cell extracts from which an inhibitor for self protein synthesis reaction is substantially excluded, having introduced therein treatment selected from supplement, storage, exchange or discharge with respect to an element selected from at least mRNA serving as a template for synthesis reaction, an energy reproduction system enzyme, a substrate, and an energy source.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.