Method for direct rescue and amplification of integrated viruses from cellular DNA of tissues
US7235393B2 · kind B2 · utility
128Cited by
5References
29Claims
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Key dates
| Filing date | Apr 22, 2003 |
| Grant date | Jun 26, 2007 |
| Priority date | — |
| Expiry date | Nov 25, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2750/14151
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method for isolating AAV viruses from cellular DNA of non-human primate (NHP) tissues by transfecting the DNA of NHP into 293 cells, rescuing the virus and amplifying it through serial passages in the presence of adenovirus helper functions is provided. Also provided are kits useful for performing this method.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.