Plant cell plastid transformation method using dual selection and producing transplastomic plants without antibiotic resistance genes
US7235711B2 · kind B2 · utility
1Cited by
4References
11Claims
0Family size
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Key dates
| Filing date | Oct 7, 2003 |
| Grant date | Jun 26, 2007 |
| Priority date | — |
| Expiry date | Aug 20, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/8274
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the field of plastid transformation. The invention provides transformation vectors and methods to obtain transplastomic plants or algae having a transformed plastid comprising the steps of introducing into plastids a recombinant nucleic acid molecule or vector, and two phases of selection with first selection phase using a non-lethal compound and a second selection phase using a lethal compound. Alternatively, the dual selection method is conducted simultaneously using a lower concentration of the lethal compound.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.