Method to measure the activation state of signaling pathways in cells
US7236888B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 21, 2001 |
| Grant date | Jun 26, 2007 |
| Priority date | — |
| Expiry date | Nov 21, 2021 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N1/02
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The activity of multiple proteins in a single living cell, portion of a cell or in a group of cells is simultaneously measured by introducing reporter molecules into the cell(s) or a portion thereof, chemically modifying the reporter(s) by the enzyme of interest, terminating the modification reactions, removing the reporter(s) and modified reporter(s), and determining the amount of enzyme activity present by measuring or comparing the amount of reporter(s) and modified reporter(s) present. By performing a series of experiments at different time points, conditions, and varieties of cell types, a database is developed for molecular cellular mechanisms in health and disease states. By exposing cells to a variety of compounds data for drug development and screening is provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.