Methods to increase nucleotide signals by Raman scattering

US7238477B2 · kind B2 · utility

Assignee

• Intel Corporation · US

Inventors

• Xing Su · Cupertino, US
• Andrew Berlin · San Jose, US
• Selena Chan · San Jose, US
• Steven J. Kirch · Pleasanton, US
• Tac-Woong Koo · South San Francisco, US
• Gabi Neubauer · Los Gatos, US
• Valluri Rao · Saratoga, US
• Narayanan Sundararajan · San Francisco, US
• Mineo Yamakawa · Campbell, US

Key dates

• Filing date: Sep 12, 2003
• Grant date: Jul 3, 2007
• Expiry date: Dec 20, 2024

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6869
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The methods and apparatus disclosed herein concern nucleic acid sequencing by enhanced Raman spectroscopy. In certain embodiments of the invention, nucleotides are covalently attached to Raman labels before incorporation into a nucleic acid. In other embodiments, unlabeled nucleic acids are used. Exonuclease treatment of the nucleic acid results in the release of labeled or unlabeled nucleotides that are detected by Raman spectroscopy. In alternative embodiments of the invention, nucleotides released from a nucleic acid by exonuclease treatment are covalently cross-linked to nanoparticles and detected by surface enhanced Raman spectroscopy (SERS), surface enhanced resonance Raman spectroscopy (SERRS) and/or coherent anti-Stokes Raman spectroscopy (CARS). Other embodiments of the invention concern apparatus for nucleic acid sequencing.