Devices and methods for biomaterial production
US7238522B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | May 27, 2004 |
| Grant date | Jul 3, 2007 |
| Priority date | — |
| Expiry date | May 27, 2024 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB03D2203/003
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
An apparatus and a method for isolating a biologic product, such as plasmid DNA, from cells. The method involves lysing cells in a controlled manner separate insoluble components from a fluid lysate containing cellular components of interest, followed by membrane chromatographic techniques to purify the cellular components of interest. The process utilizes a unique lysis apparatus, ion exchange and, optionally, hydrophobic interaction chromatography membranes in cartridge form, and ultrafiltration. The process can be applied to any biologic product extracted from a cellular source. The process uses a lysis apparatus, including a high shear, low residence-time mixer for advantageously mixing a cell suspension with a lysis solution, a hold time that denatures impurities, and an air-sparging bubble mixer that gently yet thoroughly mixes lysed cells with a neutralization/precipitation buffer and floats compacted precipitated cellular material.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.