Double ended sequencing
US7244567B2 · kind B2 · utility
328Cited by
4References
35Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Jan 28, 2004 |
| Grant date | Jul 17, 2007 |
| Priority date | — |
| Expiry date | Jul 23, 2025 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10T436/143333
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention relates to methods of sequencing DNA. More specifically, this invention relates to methods of sequencing both the sense and antisense strands of DNA through the use of blocked and unblocked sequencing primers. In brief, these methods include the steps of annealing an unblocked primer to a first strand of nucleic acid; annealing a second blocked primer to a second strand of nucleic acid; elongating the nucleic acid along the first strand with a polymerase; terminating the first sequencing primer; deblocking the second primer; and elongating the nucleic acid along the second strand.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.