Materials and methods for binding nucleic acids to surfaces
US7247721B2 · kind B2 · utility
Inventor
Key dates
| Filing date | Feb 14, 2005 |
| Grant date | Jul 24, 2007 |
| Priority date | — |
| Expiry date | Feb 14, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B40/00
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
Surfaces containing high purity silica (silicon dioxide) exhibit high loading potential for nucleic acids.Formulations containing nucleic acids and materials which mask the electrostatic interactions between the nucleic acids and surfaces are disclosed. By masking the phosphate charges of the nucleic acids, undesired interactions may be minimized or eliminated, thereby allowing the covalent bonding of the nucleic acids to the surface to proceed. The use of such formulations additionally minimizes nonspecific binding of the nucleic acids to the surface. Examples of materials to be included in such formulations include cations, xanthines, hexoses, purines, arginine, lysine, polyarginine, polylysine, and quaternary ammonium salts.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.