Muteins of human neutrophil gelatinase-associated lipocalin and related proteins

US7252998B2 · kind B2 · utility

Assignee

• Pieris AG · DE

Inventors

• Arne Skerra · Freising, DE
• Steffen Schlehuber · Ruppertsberg, DE

Key dates

• Filing date: Sep 18, 2002
• Grant date: Aug 7, 2007
• Expiry date: Nov 7, 2023

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/00
• WIPO field: Pharmaceuticals
• WIPO sector: Chemistry

Abstract

Disclosed is a method for generating a mutein of a protein selected from the group consisting of human neutrophil gelatinase-associated lipocalin (hNGAL), rat α2-microglobulin-related protein (A2m) and mouse 24p3/uterocalin (24p3), said mutein having detectable affinity to a given target. The method comprises the step of subjecting the protein to mutagenesis at one or more of the sequence positions which correspond to the sequence positions 33 to 54, 66 to 83, 94 to 106, and 123 to 136 of hNGAL, resulting in one or more mutein(s) of the protein. Also disclosed are muteins obtainable by this method.