Method for producing by cell-free protein synthesis system using thioredoxin-fused protein expression vector
US7253144B2 · kind B2 · utility
2Cited by
2References
16Claims
0Family size
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Key dates
| Filing date | Nov 19, 2004 |
| Grant date | Aug 7, 2007 |
| Priority date | — |
| Expiry date | Nov 19, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/50
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
We claim a method of expression a thioredoxin-fused membrane protein comprising expressing said thioredoxin-fused membrane protein in a cell-free protein synthesis system in the presence of a non-ionic detergent. It is preferable that the fused membrane protein which is a highly hydrophobic protein, e.g., a G protein-coupled receptor, can be produced in a state being highly soluble and capable of forming a biologically active three-dimensional structure. Also, this invention provides a recombinant polynucleotide encoding said thioredoxin-fused membrane protein which is expressed in the cell-free protein synthesis system.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.