Ligation assay
US7255994B2 · kind B2 · utility
213Cited by
2References
18Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Jun 10, 2004 |
| Grant date | Aug 14, 2007 |
| Priority date | — |
| Expiry date | Jul 12, 2025 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6827
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Disclosed are methods and kits for detecting or quantifying one or more target polynucleotide sequences in a sample. Embodiments of the invention employ a first ligation reaction, a subsequent optional amplification reaction, and a second ligation reaction. Embodiments of the invention combine the specificity of both hybridization and ligation reactions along with universal probe polynucleotide sequences to achieve specific and multiplexed detection of a plurality of target polynucleotide sequences.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.