Determination of concentration of FK778 by competitive immunoassay

US7256008B2 · kind B2 · utility

Assignee

• Abbott Laboratories · US

Inventors

• Thomas G. Spring · Highland Park, US
• Elaine M. Brate · Grayslake, US
• Shelley R. Holets-McCormack · Waukegan, US
• Rajarathnam E. Reddy · Gurnee, US
• Donald Johnson · Brookline, US
• Yon-Yih Chen · Mundelein, US
• You Pan · Hubei, CN

Key dates

• Filing date: Jan 6, 2006
• Grant date: Aug 14, 2007
• Expiry date: Jan 6, 2026

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N33/9493
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods and kits for measurement of concentration of FK778 in a biological sample by means of an immunoassay, preferably a competitive immunoassay. In one aspect, the method and kit involve the use of (a) an antibody to FK778 conjugated to a label, e.g., an acridinium label, (b) an antibody to FK778 not conjugated to a label, (c) a solid phase containing an antibody to a first hapten, e.g., a fluorescein hapten, and (d) a bihapten comprising a first hapten and FK778 or an analogue of FK778, e.g., a bihapten comprising a fluorescein hapten and a FK778 hapten. In another aspect, the method and kit involve the use of (a) antibody to FK778, (b) a bihapten comprising FK778 or an analogue of FK778 and a first hapten, e.g., a bihapten comprising the fluorescein hapten and the hapten of FK778 or an analogue of FK778, and (c) a pretreatment reagent.