Methods and compositions for detecting target sequences
US7256020B2 · kind B2 · utility
31Cited by
19References
13Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Feb 3, 2003 |
| Grant date | Aug 14, 2007 |
| Priority date | — |
| Expiry date | Feb 3, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2710/16111
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
The present invention relates to compositions and methods for the detection and characterization of nucleic acid sequences and variations in nucleic acid sequences. The present invention relates to methods for forming a nucleic acid cleavage structure on a target sequence and cleaving the nucleic acid cleavage structure in a site-specific manner. For example, in some embodiments, a 5′ nuclease activity from any of a variety of enzymes is used to cleave the target-dependent cleavage structure, thereby indicating the presence of specific nucleic acid sequences or specific variations thereof.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.