Enzymes for the production of 2-keto-L-gulonic acid

US7256027B1 · kind B1 · utility

Assignee

• Rutgers, the State University of New Jersey · US

Inventors

• Robert A. Lazarus · Millbrae, US
• Mark Hurle · Norristown, US
• Stephen Anderson · Princeton, US
• David Powers · Fairfax, US

Key dates

• Filing date: Jun 15, 1999
• Grant date: Aug 14, 2007
• Expiry date: Jun 15, 2019

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N9/0006
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Mutants of 2,5-diketo-D-gluconic acid reductase A, an enzyme used to produce 2-keto-L-gulonic acid, a precursor of ascorbic acid (vitamin C) are prepared by site-directed mutagenesis. These mutants have increased catalytic activity, increased expression levels, and/or enhanced temperature stability.