Patent · US Expired

Intrabodies with defined framework that is stable in a reducing environment and applications thereof

US7258986B2 · kind B2 · utility

13Cited by

1References

6Claims

0Family size

Assignee

ESBATech · CH

Inventors

Adrian Auf Der Maur · Dietikon, CH
Alcide Barberis · Bioggio, CH
Dominik Escher · Kreuzlingen, CH

Key dates

Filing date	Dec 18, 2000
Grant date	Aug 21, 2007
Priority date	—
Expiry date	Dec 18, 2020

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC07K2319/00
WIPO fieldPharmaceuticals
WIPO sectorChemistry

Abstract

A method for the isolation of CDRs in a defined framework that is stable and soluble in reducing environment is described as well as thus obtainable scFv. Starting from such scFv with defined framework a scFv library can be generated wherein the framework is conserved while at least one complementary determining region (CDR) is randomized. Such library, e.g. in yeast cells, is suitable for screening for antibody/CDR-interactions or for screening for antibodies.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.