Alleviation of non-specific binding in microarray assays
US7258990B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 19, 2004 |
| Grant date | Aug 21, 2007 |
| Priority date | — |
| Expiry date | Mar 15, 2025 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/543
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A post-incubation treatment is employed to effectively remove targets, such as proteins/protein complexes, or other label-bearing moieties that may non-specifically bind to a microarray substrate during a binding assay. Following incubation, a one-step wash is carried out with a liquid containing digester, e.g., a digestive enzyme (protease) or lysosome, which is effective to remove non-specifically bound targets or at least labeled portions of such targets from the substrate. Proteases are bound to or coated onto large molecules or onto solid particles of such a size such that they are prohibited from entering the porous surfaces of 3-D hydrogel microspots and are unable to reach and digest labeled target-probe complexes that are disposed within such porous hydrogel microspots. Digested segments of such protein which contain labels (or of essentially the entire protein) are carried away in the wash liquid and thus are not present to create background noise during imaging.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.