Methods of increasing protein expression levels
US7258997B2 · kind B2 · utility
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4References
5Claims
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Key dates
| Filing date | Nov 21, 2002 |
| Grant date | Aug 21, 2007 |
| Priority date | — |
| Expiry date | Feb 13, 2023 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY10S930/144
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to methods of increasing protein expression levels whereby at least one amino acid in a protein amino acid sequence is substituted for the amino acid, proline. Preferably, the substitution occurs within 15 amino acids, more preferably within 10 amino acids and most preferably within 5 amino acids of a cysteine amino acid residue. The present invention not only includes methods for polypeptides with proline substitutions, but also polynucleotides with codon substitutions for which a codon for any amino acid, except proline, is substituted for a codon encoding for proline.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.