Methods for eliminating mannosylphosphorylation of glycans in the production of glycoproteins
US7259007B2 · kind B2 · utility
37Cited by
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5Claims
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Key dates
| Filing date | Dec 22, 2004 |
| Grant date | Aug 21, 2007 |
| Priority date | — |
| Expiry date | Dec 22, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P21/005
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the elimination of mannosylphosphorylation on the glycans of glycoproteins in the yeast genus Pichia. The elimination of mannosylphosphorylated glycoproteins results from the disruption of the PNO1 gene and the newly isolated P. pastoris MNN4B gene. The present invention further relates to methods for producing modified glycan structures in host cells that are free of glycan mannosylphosphorylation.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.