Enhanced production of taxol and taxanes by cell cultures of Taxus species
US7264951B1 · kind B1 · utility
Assignee
Inventors
Key dates
| Filing date | May 22, 1998 |
| Grant date | Sep 4, 2007 |
| Priority date | — |
| Expiry date | Jan 13, 2019 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P15/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
This invention provides methods whereby taxol, baccatin III, and other taxol-like compounds, or taxanes, can be produced in very high yield from all known Taxus species, e.g., brevifolia, canadensis, cuspidata, baccata, globosa, floridana, wallichiana, media and chinensis. Particular modifications of culture conditions (i.e., media composition and operating modes) have been discovered to enhance the yield of various taxanes from cell culture of all species of Taxus. Particularly preferred enhancement agents include silver ion or complex, jasmonic acid (especially the methyl ester), auxin-related growth regulators, and inhibitors of the phenylpropanoid pathway, such as 3,4-methylenedioxy-6-nitrocinnamic acid. These enhancement agents may be used alone or in combination with one another or other yield-enhancing conditions. While the yield of taxanes from plant cell culture of T. chinensis is particularly enhanced by use of one or more of these conditions, yield of taxanes for all Taxus species has been found to benefit from use of these conditions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.