Rapid extraction of RNA from cells and tissues
US7267950B2 · kind B2 · utility
4Cited by
17References
3Claims
0Family size
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Key dates
| Filing date | May 1, 2003 |
| Grant date | Sep 11, 2007 |
| Priority date | — |
| Expiry date | Mar 22, 2024 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
A method of extracting RNA from biological systems (cells, cell fragments, organelles, tissues, organs, or organisms) is presented in which a solution containing RNA is contacted with a substrate to which RNA can bind. RNA is withdrawn from the substrate by applying negative pressure. No centrifugation step exceeds thirty seconds. Preferably, the RNA is diluted prior to filtration and one or more wash steps can be used to remove interferents. In one such method, except for DNA shearing and drying steps, centrifugation is not undertaken during the extraction step.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.