Nucleic acid probe and novel method of assaying nucleic acid using the same
US7273700B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Mar 27, 2002 |
| Grant date | Sep 25, 2007 |
| Priority date | — |
| Expiry date | Feb 12, 2023 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6818
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A novel nucleic acid probe for nucleic acid determination includes a single-stranded nucleic acid labeled with plural fluorescent dyes containing at least one pair of fluorescent dyes to induce FRET, the pair of fluorescent dyes including a fluorescent dye (a donor dye) capable of serving as a donor dye and a fluorescent dye (an acceptor dye) capable of serving as an acceptor dye, in which the nucleic acid probe has such a base sequence and is labeled with the fluorescent dyes so that the fluorescence intensity of the acceptor dye decreases upon hybridization with a target nucleic acid. A novel nucleic acid determination method uses the probe. The probe and method can determine one or more types of target nucleic acids in an assay system in parallel using a simple apparatus.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.