Method of reversibly disrupting a conjugate comprising a biotin compound and a biotin-binding compound
US7279285B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Feb 1, 2002 |
| Grant date | Oct 9, 2007 |
| Priority date | — |
| Expiry date | Mar 21, 2023 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/54393
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention relates to a method of disrupting a conjugate comprising a biotin compound and a biotin-binding compound, said method comprising the step of contacting said conjugate with an effective amount of a substantially aqueous solution under conditions such that the conjugate is disrupted, preferably reversibly disrupted, thereby forming a biotin compound and a biotin-binding compound. In particular, the invention relates to a method of reversibly releasing a biotinylated moiety from a streptavidin (or avidin) coated support. Uses of the methods in techniques of detection, identification, determination, purification, separation and/or isolation of target proteins or nucleic acid molecules are also encompassed by the present invention.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.